The best cut-off values (sensitivity and specificity) for predicting SPTD at <34 weeks of gestation after cerclage were 0.502 ng/ml for AF APRIL (85% sensitivity and 60% specificity), 2.996 ng/ml for AF IL-8 (85% sensitivity and 55% specificity), 0.170 ng/ml for AF MIP-1α (80% sensitivity and 60% specificity), 2.548 μg/ml for AF S100 A8/A9 (80% sensitivity and 85% specificity), and 1.003 μg/ml for AF TIMP-1 (75% sensitivity and 80% specificity) (Table 5). The gene discussed is CXCL8; the disease is atrial fibrillation.