In conclusion, using protein–antibody microarray technology, we have identified several novel biomarkers—APRIL, IL-8, MIP-1α, S100 A8/A9, and TIMP-1, which are associated with the development of SPTD at <34 weeks of gestation after rescue cerclage in the AF from women with cervical insufficiency. Here, TIMP1 is linked to atrial fibrillation.