In this report, we have studied the effect that VRK1 depletion has in glioblastoma cells, based on the observation that VRK1 is a chromatin kinase which regulates several steps in the DDR (Sanz-Garcia et al., 2012; Salzano et al., 2014; Salzano et al., 2015; Monsalve et al., 2016) and impairs the NHEJ pathway (Sanz-Garcia et al., 2012). This evidence concerns the gene VRK1 and glioblastoma.