IFNG and infection: In the early stage of mouse infection, macrophages allow the survival and replication of Brucella. With prolonged infection time, after the adaptive immune response is gradually established, macrophage antibacterial pathway components such as RNIs and ROIs are activated by IFN-γ and TNF-α, and activated macrophages then become the primary source of Brucella elimination from infected mice (Dornand et al., 2002; Dorneles et al., 2015).