To further explore whether 1g (10 μM)-stimulated ROS production of colon cancer cells can cause their endogenous apoptosis by ER-stress, the gene expression of CHOP and GRP78 was quantified by RT-PCR in HCT116 cells at various times (0 to 8 h) (Figures 6A, B), and CHOP and GRP78 gene expression in 1g and Cur-treated HCT116 cells for 8 h (Figures 6C, D). The gene discussed is HSPA5; the disease is malignant colon neoplasm.