This important biological characteristic of LAT1 was also substantiated by our present study, i.e., 40-kDa LAT1 proteins were detected under non-reducing conditions in WB analysis (Figure 1E and 6E), and anti-LAT1 mAb reacted with living HEK293 cells and SW1116 colon cancer cells whose CD98hc gene was completely disrupted by the CRISPR/Cas 9-based KO (Figure 6D). This evidence concerns the gene SLC3A2 and malignant colon neoplasm.