Fortunately, more and more studies have found breakthroughs to improve the efficacy of immunotherapy and reduce side effects, such as thioredoxin, FATP2, bacterial transplantation, etc. Moreover, effective indicators such as tumor mutation load index and tumor microenvironment scoring system can be used to screen the population that can benefit from immunotherapy and evaluate the efficacy. The gene discussed is TXN; the disease is neoplasm.