To our best knowledge, our study was the first to confirm that in NSCLC tissues, KIF3C was highly expressed, and its high expression was significantly associated with the patient’s adverse prognosis; furthermore, KIF3C overexpression dramatically promoted NSCLC cell proliferation, migration, and invasion, and its depletion repressed these malignant phenotypes of NSCLC cells. The gene discussed is KIF3C; the disease is non-small cell lung carcinoma.