KIF3C and non-small cell lung carcinoma: Besides, to validate whether miR-186-3p was involved in NSCLC progression via targeting miR-186-3p, we co-transfected pcDNA-KIF3C+miR-186-3p mimics into H226 cells, and co-transfected si-KIF3C+miR-186-3p inhibitors into A549 cells, and the transfection was confirmed by Western blotting to be successful (Figure 5a).