Gene BAMBI (p = 0.024) and gene ALOX5AP (p = 0.001) were top upregulated genes in osteosarcoma while gene WDR1 (p = 0.042), gene PML (p = 0.012), gene MAP3K5 (p = 2.60E − 05), gene GNPDA2 (p = 0.027), gene CCL5 (p = 7.37E − 08), and gene MAOA (p = 9.08E − 07) were top upregulated genes in normal bone sample. Here, MAP3K5 is linked to osteosarcoma.