The expression of gene WDR1 (p = 0.006), PML (p = 0.005), MAP3K5 (p = 0.010), and GNPDA2 (p = 0.009) were significantly higher in normal bone sample while in osteosarcoma samples, the expression of BAMBI (p = 0.006) was significantly higher. The gene discussed is MAP3K5; the disease is osteosarcoma.