Study has reported that the demethylation of the upstream promoter of Shc3 increased the expression of Shc3 to induce EMT and promote HCC cell metastasis.60 Melanoma antigen (MAGE)-encoding genes were expressed in advanced gastric cancer via demethylation of promoter CpG islands.61 There are two main ways of DNA demethylation. This evidence concerns the gene SHC3 and hepatocellular carcinoma.