Notably, deregulation of HSF1 targets was identified for a stringent direct target gene set regulated by HSF1 binding (ChiP-seq/RNA-seq) after HSF1 knockdown in breast cancer cells46 (Fig. 6c and Supplementary Fig. 6a), as well as for a second independent target gene set generated from HS treated cancer cells64 (Supplementary Fig. 6b, c). The gene discussed is HSF1; the disease is breast cancer.