PRF1 and melanoma: Similarly, CD56dimCD16+ NK cells also exhibited reduced DNAM1 as well as GzmB and perforin expression (Supplementary Fig. S3D and S3G) while modest changes in the activating receptors NKG2D and NKp46 expression were found in CD56dimCD16− NK cells from advanced melanoma compared with HD (Supplementary Fig. S3E and S3H).