Lower transcript levels of MTHFD1 and PYGB genes determined poor overall survival in patients with HNSCC (p-value = 0.006 and p-value = 0.0312, respectively) using the HNCDB (Fig. 5B; supplemental Table S20), while lower gene expression of CANT1 and GOT1 was associated with reduced metastasis-free survival and overall survival, respectively, according to PROGgeneV2 tool (p-value = 0.0109 and p-value = 0.0410) (Fig. 5C; supplemental Table S20). This evidence concerns the gene PYGB and head and neck squamous cell carcinoma.