Further evaluation of the six serum EV‐derived lncRNAs in the test cohort (n = 44) consisting of NL (n = 8), CH (n = 7), LC (n = 10), mUICC I/II HCC (n = 9), and mUICC III/IV HCC (n = 10) identified four lncRNAs—serum EV‐derived DLEU2 (EV‐DLEU2), HOTTIP (EV‐HOTTIP), MALAT1 (EV‐MALAT1), and SNHG1 (EV‐SNHG1)—as significantly discriminatory for the HCC and non‐HCC samples. The gene discussed is HOTTIP; the disease is hepatocellular carcinoma.