We found that DEGs associated with membrane formation, transfer, and autophagy related pathway were significantly enriched in the RGNNV infection group, and high levels of protein tyrosine phosphatase receptor type F (ptprf), vasoactive intestinal peptide receptor 2 (vipr2), heat shock protein 90 (hsp90), lysine (K)-specific demethylase 6B (kdm6b), rho-GTPase activating protein 6 (arhgap6), and solute carrier family 6 member 6 (slc6a6) were detected (Fig 8A and 8B). The gene discussed is KDM6B; the disease is infection.