Hence, we were promoted to evaluate the TLS score by the examination of marker gene expression including CCL19, CCL21, CXCL13, CCR7, SELL, LAMP3, and CXCR4 and observed similar TLS genes score in tumors comparing with adjacent tissues, which implied the tertiary lymphoid structures in CRC tumor was intact (Figure 6C). This evidence concerns the gene CCL21 and colorectal carcinoma.