To validate the scRNA‐seq results, we analyzed independent gallbladder adenocarcinoma and adjacent normal tissues by immunohistochemistry (IHC) staining (Figures 4F and S3E) and found a significant increase of FOXP3+ or CD4+ cells and a reduction of CD8A+ cells in tumors (Figure 4F). The gene discussed is CD4; the disease is gallbladder adenocarcinoma.