CHAT and Alzheimer disease: Co‐immunofluorescence staining and quantitative analysis revealed that pTau was increased in MS cholinergic neurons of the AD mice (Figure 2A–D), ∼80% of ChAT‐positive neurons were colocalized with pT205 and pT231 in 10‐month‐old 3xTg (Figure 2A,B) and 5xFAD (Figure 2C,D) mice.