To investigate the function of CLTC in osteosarcoma cells, we utilized two independent siRNAs to down‐regulate the expression of CLTC. Western blotting (WB) and quantitative real‐time PCR (qRT‐PCR) assays showed that both CLTC siRNAs significantly decreased CLTC expression in the osteosarcoma cell lines (Figure 2A–C). This evidence concerns the gene CLTC and osteosarcoma.