The CpG sites located in or near the CpG island (island, N shore and S shore) covering the TSS of CD40 (12 CpG sites) were significantly hypermethylated in CRC tissues compared with the adjacent mucosa (Fig. 6C), and except for cg24575067, their methylation levels were negatively correlated with CD40 expression (Fig. 6D). Here, CD40 is linked to colorectal carcinoma.