Similarly, to study the effect of Prox1 on the invasive ability of A549 and H446 lung cancer cells, this study used Transwell experiment to detect the change of the invasive ability of A549 and H446 cells overexpressing Prox1 after plasmid transfection and A549 and H446 cells with low expression of Prox1 after transfection of siRNA. The gene discussed is PROX1; the disease is lung carcinoma.