Despite no change in the expression of the TLRs (i.e., TLR4, and TLR2), we may speculate that the downregulation in the expression of fundamental markers associated with adipocyte differentiation (Pparg, Cebpa), may explain adipocyte death, recruitment of immune cells, and production of proinflammatory cytokines, thereby triggering adipose tissue inflammation and insulin resistance in db/db mice. Here, TLR4 is linked to Insulin resistance.