CBLN2 and cancer: We also found that CBLN2 and TMEM220 expression were down-regulated and SLCO4C1 expression was up-regulated in SW480 cells with RBM47 knockdown, which suggested that CBLN2, SLCO4C1, and TMEM220 were involved in the development of COAD under the regulation of RBM47. A large number of previous studies have shown that mRNA expression levels are regulated by promoter methylation of SLCO4C1 in cancers, such as colorectal cancer [33], prostate cancer [34] and head and neck cancers [35].