The accompanying studies show that deletion of Kiss1 in the ARC results in a significant decrease in LH pulse frequency in both sexes, affecting gonadal development and gametogenesis, and resulting in infertility in Pdyn-Cre/Kiss1fl/fl KO females and subfertility (ranging from infertility in one mouse to fewer litters in other mice) in Pdyn-Cre/Kiss1fl/fl KO males. The gene discussed is PLOD1; the disease is Infertility.