Based on the recommendation by Nor Salmah B, Mardiana AA, Ruzi H, Rose A, Noor Kaslina MK et al.,(2018), for tissue optimization in lung carcinoma cases, they proposed multiple levels of the tissue as follows; Level-1 for H&E, followed by two unstained slides on poly-l-lysine, Level-2 H&E, another two unstained slides on poly-l-lysine, six unstained slides for EGFR gene mutations testing, and Level-3 for H&E. Here, EGFR is linked to lung carcinoma.