Most breast cancer samples in this study, regardless of their subtypes, displayed both cytoplasmic and nuclear expression patterns of TIMAP. This is in agreement with previous studies that showed TIMAP can be localized to the plasma membrane, cytoplasm and the nucleus (Cao et al., 2002; Kim et al., 2005; Li et al., 2007; Csortos et al., 2008; Boratkó et al., 2015; Boratkó and Csortos, 2017); and this is attributed to its N-terminal nuclear localization signal and the C-terminal CAAX domain (Cao et al., 2002) that allow TIMAP to shuttle between these cell compartments. The gene discussed is PPP1R16B; the disease is breast cancer.