In the current study, we investigated TIMAP protein expression in 159 breast cancer tissue samples categorized into the following subtypes; Human epidermal growth factor receptor 2 (HER2)-only, Luminal A, Luminal B, and triple negative, by immunohistochemistry (IHC) using tissue microarray (TMA) and correlated its protein expression with different clinicopathological features and overall survival rate. Here, PPP1R16B is linked to breast cancer.