To further investigate the effect of CS-RC nanoparticles on cytokine and chemokine expression in the asthma model, ELISA was adopted for measuring the expression of IL-4, IL-17, IL-17F, IL-6, IL-23, MIP-1α, MIP-2, and IFN-γ in BALF (Figure 3). This evidence concerns the gene IL4 and asthma.