Next, cell proliferation abilities and viability were monitored by using the CCK-8 assay and trypan blue staining assay for 0 h, 12 h, 24 h, 36 h and 48 h, and the results in Figure 2(d,e) showed that overexpression of CXCR4 promoted cell proliferation in the ccRCC cells, while CXCR4 ablation had opposite effects to hinder RCC cell growth (Figure 2(d,e)) and inhibited cell viability (figure 2(f,g)). This evidence concerns the gene CXCR4 and nonpapillary renal cell carcinoma.