Finally, we found that the expression of TPM1 occurred in NK cells, non-classical monocytes, naive CD4 + T cells, native T-reg cells, memory CD4 T-cell TFH, and memory CD4 T-cell Th2, and the findings indicated that TPM1 might possess immunomodulatory functions; therefore, further exploration of the common target genes of ICB and EMT-related gene expression in colon cancer is necessary. Here, TPM1 is linked to malignant colon neoplasm.