Subsequently, we further shortlisted the 63 genes with high PSI in the tumor samples, preferred locations of alternative exon in the coding region, appropriate length of the alternative exon (30–300 bp), and successful validation of AS events using reverse transcription‐PCR (RT‐PCR), which revealed GOLIM4 as the gene of interest (Figure 1C). The gene discussed is GOLIM4; the disease is neoplasm.