Of the total ten w8 genes altered by everolimus, 70% (GINS2, CCDC130, PRR22, CYP4F2, PGLS, LY9, and BCHE) have been identified as essential ccRCC genes in a CRISPR genome-wide loss-of-function screen29 (Figure S2D). This evidence concerns the gene PGLS and nonpapillary renal cell carcinoma.