To assess whether the ACSNI-predicted mTOR signaling captured KLF and EPAS1 signal, we first defined KLF6 and EPAS1 signals as differentially expressed (DE) genes from publicly available gene perturbation datasets: (1) KLF6 knockout (KLF6-KO, DE = 1,378 genes) and (2) EPAS1 knockout (EPAS1-KO, DE = 303 genes) in the 786 ccRCC cell line.23 Here, KLF6 is linked to nonpapillary renal cell carcinoma.