And the results showed that demethylation of PLOD2 mRNA induced by dm6ACRISPR led to a decreased cell proliferation rate in the 786-O and OSRC RCC cells as compared with that of non-targeted gRNA combined with dCas13b-ALKBH5 (Figures 4A–E). The gene discussed is ALKBH5; the disease is renal cell carcinoma.