To further select the TFs with the highest prognostic value, a multivariate Cox regression analysis was performed for investigating its impact, and six hub TFs were selected for establishing a risk model for CRC patients, including EST2, HSF4, SALL1, HEYL, SIX2, and ZNF26. The hazard ratios of the four genes, HSF4, HEYL, SIX2, and ZNF26, were positive, which show their negative match with the prognosis. This evidence concerns the gene ZNF26 and colorectal carcinoma.