LncRNA Xist, which participated in osteosarcoma development processes, including cell proliferation, migration, invasion, EMT, and apoptosis, is involved in gene regulation through a variety of mechanisms, primarily by functioning as a miRNA sponge and via interacting with its targets (Li et al., 2017; Wang et al., 2019f; Han and Shen, 2020), such as miR-153/SNAI1 pathway (Wen et al., 2020), EZH2, PUMA, and NF-kB (Xu T. et al., 2017; Gao et al., 2019). The gene discussed is NFKB1; the disease is osteosarcoma.