Although PQR620 disrupted assembles of mTORC1 (mTOR-Raptor) and mTORC2 (mTOR-Rictor-Sin1), and inhibited Akt-S6K1-S6phosphorylations in NSCLC cells, our results suggested that mTOR-independent mechanisms also participated in PQR620-induced NSCLC cytotoxicity. The gene discussed is AKT1; the disease is non-small cell lung carcinoma.