In other primary (pNSCLC-2 and pNSCLC-3) and established (A549 and NCI-H1944) NSCLC cells, treatment with PQR620 (300 nM) induced caspase-3 activation (Figure 2I), mitochondrial depolarization (JC-1 green monomers intensity increase, Figure 2J), and increased ratio of apoptotic nuclei (Figure 2K), confirming apoptosis activation. This evidence concerns the gene CASP3 and non-small cell lung carcinoma.