In order to get a better understanding of the roles of the NUF2 gene in NSCLC, we perform expression profiling, prognosis valuation, DNA methylation, gene mutation, immune infiltration, clinic pathologic association significance of the NUF2 gene with the public-accessible cancer genomics database and also validated NUF2 expression by reverse transcription polymerase chain reaction (RT-PCR) in NSCLC. The gene discussed is NUF2; the disease is non-small cell lung carcinoma.