MTOR and esophageal squamous cell carcinoma: Additionally, to examine whether Ipriflavone could inhibit mTOR signaling pathway-dependent ESCC cell growth and early cell apoptosis, ESCC cells expressing shmTOR #3 or shControl were treated with Ipriflavone for 48h (MTT assay; Figure 5D), 7 days (Foci formation; Figure 5E), 2 weeks (Soft agar assay; Figure 5F) or 72 h (Supplemental Figure 5).