In the cohort of patients diagnosed with squamous cell carcinoma, worse RFS was observed in patients with elevated preoperative serum CYFRA21-1(P=0.011), NSE(P=0.0041), CA125(P=0.0046), CA153(P=0.002) and CA199(P=0.014). The gene discussed is MUC16; the disease is squamous cell carcinoma.