Result that ALDH1A3 mRNA was significantly upregulated in TSC2-/- S102 and patient derived LAM lung cell lines that leads to mTOR activation and downregulation of RARβ expression (6) was confirmed by immunofluorescent staining (Figure 1D) and western blotting (Figure 1E). This evidence concerns the gene TSC2 and lymphangioleiomyomatosis.