TLE6 and Infertility: In addition, we used light microscopy for five consecutive days to observe the development and morphology of the embryos from one patient (II-4 in family 1) who carried the homozygous TLE6 frameshift variant c.1631_1632delCA (p.Pro544Argfs∗5) and found that three embryos had a high percentage of fragmentation during culture, whereas the other five embryos were arrested on the third day, showing similar phenotype in embryogenesis between infertile women carrying TLE6 variants and Tle6Null female mice.