Notably, mRNA levels of caspase-1 and IL-1β were found to be significantly increased in PBMCs from patients with CRMO at active and remission stages compared to those in healthy controls, and the expression of NLRP3, ASC, caspase-1, and IL-1β was also detected in bone tissues of patients with CRMO (197). This evidence concerns the gene PYCARD and chronic recurrent multifocal osteomyelitis.