In addition, the confocal laser microscopy results showed that E. coli infection significantly increased the expression of p-p38, p-p65, and TLR4, while pretreatment with MPX significantly decreased the expression of p-p38, p-p65, and TLR4 (Figure 1F), indicating that MPX could inhibit the release of inflammatory cytokines by reducing the phosphorylation of p38 and the activation of p65 and TLR4. The gene discussed is TLR4; the disease is escherichia coli infection.