As shown in Fig. 7B, C26 CM upregulated Ddit4, Akt1, and mTOR expression and downregulated phospho-4E-BP1, and phospho-p70S6K in C2C12 compared with CM of CT26, another colon cancer cell line that could not lead to cancer cachexia. This evidence concerns the gene RPS6KB1 and malignant colon neoplasm.