The results of GTT and the ITT assays showed that HFD-influenced glucose tolerance and insulin sensitivity were significantly improved in the mice treated with hAMSCs-CM compared with the mice treated with SFD (Fig. 2B, C), further confirming that HFD-induced obesity was accompanied with hyperglycemia and the anti-obesity of hAMSCs-CM might be related with improvement of HFD-induced glucose tolerance and insulin resistance. The gene discussed is INS; the disease is obesity due to melanocortin 4 receptor deficiency.