IGF1 and neoplasm: Moreover, to clarify whether the PI3K/Akt signaling pathway directly mediates the effects of ARHGAP10 on cell metastasis, A549 and NCI-H1299 cells treated with or without ARHGAP10 were exposed to insulin-like growth factors-1 (IGF-1) (100 ng/mL), an activator of PI3K/Akt pathway in tumor progression [16, 17], and evaluated the expression level of EMT biomarkers by Western blot analysis.