To further elucidate subcellular localization and expression of ARHGAP10 in NSCLC cell, we performed immunofluorescence assay in A549 and NCI-H1299 cell lines, confocal micrographs displayed that ARHGAP10 localized to the nuclei and cytoplasm region (Fig. 2e, f). The gene discussed is ARHGAP10; the disease is non-small cell lung carcinoma.