Compared to the controls, ERMS incidence (palpable and non-palpable ERMS) was significantly higher in tamoxifen-treated Ptch+/−HRasfl/+Myf5CreER/wt and Ptch+/−KRasfl/+Myf5CreER/wt mice (Fig. 5A, B, Table 1). The gene discussed is PTCH1; the disease is embryonal rhabdomyosarcoma.