Both thin sections and PDS cubes (2 × 3 × 3 mm) showed gene expression changes with upregulation of pluripotency (POU5F1, SOX2, NANOG and NEAT1) and EMT markers (VIM and SNAI1), but decrease of proliferation marker genes (MKI67 and CCNA2), and the differentiation marker CDH1 as well as the breast cancer stem cell (BCSC) gene ABCG2. The gene discussed is NANOG; the disease is breast carcinoma.