Next, a luciferase reporter assay was conducted to evaluate the functional impact of the 29940 G-to-C mutation on NLRP3 expression and showed that the allele-specific luciferase vector with the sepsis risk-associated G allele exhibited significantly higher activities than the C allele, which corroborated the findings of several previous studies22,37. Here, NLRP3 is linked to Sepsis.