In western blotting, suppressed expression of E-cadherin (the marker of epithelial cell) and upregulated expression of vimentin, α-SMA, and N-cadherin (markers of mesenchymal cells) were detected (Fig. 4C) meanwhile opposite expressions of these protein markers were detected in MG63 cells with silent CT45A1 which suggested that CT45A1 could induce EMT in osteosarcoma cells (Fig. 4E). This evidence concerns the gene ACTA1 and osteosarcoma.