CT45A1 and osteosarcoma: To confirm the role of CT45A1 in osteosarcoma further, we established osteosarcoma cells with CT45A1 overexpression or silencing based on the basal expression of CT45A1 in osteosarcoma cell lines (Supplemental Fig. 1A, B), and the indicated levels of CT45A1 were detected by western blotting assay (Supplemental Fig. 1C, E) and qRT-PCR (Supplemental Fig. 1D, F).