In this study, the apoptotic activities of ACRH, QRF, and BQ at their respective concentrations (0.05, 0.5, and 5 μg/mL) on IL-1β-induced HFLS-RA cell apoptosis were assayed in a flow cytometer using FITC Annexin V Apoptosis Detection Kit I. Figure 5 illustrated the cell distribution of IL-1β-HFLS-RA after 24 h treatment with the respective samples. The gene discussed is ANXA5; the disease is rheumatoid arthritis.