Incubation of isolated primary lung fibroblasts with CM from Met-1, a PyMT-derived breast carcinoma cell line (Borowsky et al., 2005), or from 4T1 cells, a model of triple-negative breast cancer, indicated that tumor-derived factors activated multiple CAF-associated functions including enhanced motility in wound healing assay (Figure 5—figure supplement 1[1–4]) and increased contraction of collagen gel matrices (Figure 5—figure supplement 1[5–7]). Here, GZMM is linked to breast carcinoma.